Home

Biology (nonmajor)
General Biology I
General Biology I Lab
General Biology II
General Biology II Lab
Microbiology
Microbiology Lab
Genetics

Useful Freeware

last updated: 19 Aug 2015

This course is a one credit hour lab course in microbiology for nursing and allied health majors. The lab portion meets three hours a week.  The course will cover numerous aspects of the microbial world; however, it will concentrate on human-microbe interactions.

The experiment numbers refer to the exercises in the Collin County Community College Spring 2015 Lab Manual. If this link does not work, go to this website and download the most recent manual for BIOL 2420 in the frame on the left.

For more detailed information, examine the syllabus and course policies. For advice on how to succeed in this course, and in all other courses, take a peek at the lab hints. Bloom's taxonomy of understanding will help you differentiate the increasing levels of insight of a topic.

Other important documents
Syllabus
Lab Schedule

To view any of these documents, you need Adobe Reader. If you do not have Adobe Reader, you can download and install it for free here


Dates
Topic		 Experiment
Number
08/17 Introduction, Safety Policy, Contact Lens Policy
Manipulation of Living Cultures		 Handout(2)
6,7
08/24 Manipulation of Living Cultures (finish)
Microscopy with the Olympus CH-22
Image of Olympus Microscope
Exercise 1 - MICROSCOPY
Concepts in Microscopy
     Magnification
     Resolution
     Field of View
     Depth of Field
     Contrast		 6,7
1,Handout
Handout
Handout
08/31 Simple Staining
Negative Staining
Exercise 2 - BACILLUS ISOLATION from SOIL		 1

Handout
09/14 Gram Staining
Acid-Fast Staining
Exercise 2 - BACILLUS ISOLATION from SOIL (continue)
Microscopy of Living Samples		 2
4
Handout
09/21 Endospore Staining
Exercise 2 - BACILLUS ISOLATION from SOIL (finish)		 5
Handout
09/28 QUIZ #1
Selective & Differential Media
13
10/05 Selective & Differential Media (finish)
Temperature Requirements
Oxygen Requirements
Carbohydrate Fermentation
Exercise 4 - DEVELOPMENT of an IDENTIFICATION KEY		 13
10
11
28
Handout
10/12 No Class - Fall Break
10/19 Temperature Requirements
Oxygen Requirements (finish)
Carbohydrate Fermentation (finish)
IMViC Tests
Exercise 4 - DEVELOPMENT of an IDENTIFICATION KEY (cont.)		 10
11
28
28
Handout
10/26 IMViC Tests (finish)
Hydrolytic Enzymes
Nitrate Reduction Test
Exercise 4 - DEVELOPMENT of an IDENTIFICATION KEY (cont.)
Exercise 5 - ENTERIC IDENTIFICATION		 28
20,25,28
20
Handout
Handout
11/02 Hydrolytic Enzymes (finish)
Nitrate Reduction Test (finish)
Catalase & Oxidase Tests
Exercise 4 - DEVELOPMENT of an IDENTIFICATION KEY (finish)
Exercise 5 - ENTERIC IDENTIFICATION (finish)		 20,25,28
20
22
Handout
Handout
11/09 Exercise 4 - CREATION of the DICHOTOMOUS KEY
Antisepsis
Antibiotics
Exercise 6 - STAPHYLOCOCCUS ISOLATION from SKIN
14
15
Handout,25
11/16 Antisepsis (finish)
Antibiotics (finish)
Exercise 6 - STAPHYLOCOCCUS ISOLATION from SKIN (cont.)
Exercise 7 - IDENTIFICATION of UNKNOWN		 14
15
Handout,25
Handout,A,B
11/23 Exercise 6 - STAPHYLOCOCCUS ISOLATION from SKIN (finish)
Exercise 7 - IDENTIFICATION of UNKNOWN (finish)		 Handout,25
Handout,A,B
11/30 QUIZ #2




Important College Deadlines
08/24 Last Day of Add/Drop
08/31 Last Day to Drop with 80% Refund
09/14 Last Day to Withdraw with Refund
10/12 Last Day to Withdraw with a W